Table I.
3ISG sequence #s | 3ISG PDB #s | Function/comment |
---|---|---|
Pro44 | Pro65 | |
Ser46a | Ser67b | Nucleophile that attacks β-lactam |
Thr47 | Thr68b | |
Phe48 | Phe69b | |
Lys49a | Lys70b | General base |
Ser93a | Ser115c | |
Gly106 | Gly128 | |
Tyr119 (some Phe) | Tyr141d | Ω-loop Class D motif |
Gly120a | Gly142d | Ω-loop Class D motif |
Asn121 | Asn143d | Ω-loop Class D motif |
Trp138a | Trp160e | Interacts with general base |
Ile145 | Ile167 | Val makes up the remainder |
Leu162 | Leu184 | Val, Phe make up the remainder |
Lys190a | Lys212f | Likely proton donor |
Gly192a | Gly214f | Mainchain amide contributes to oxyanion hole |
Trp205a | Trp228 | Part of hydrophobic core |
Gly208a | Gly231 | |
Phe220a | Phe243 |
aStrictly conserved.
bSTFK (Paetzel et al., 2000).
cSXV (Paetzel et al., 2000).
dYGN (Paetzel et al., 2000).eBaurin et al. (2009).
fKTG family signatures (Paetzel et al., 2000).