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. 1998 Jul 15;12(14):2208–2221. doi: 10.1101/gad.12.14.2208

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Copyright © 1998, Cold Spring Harbor Laboratory Press

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Time course analysis of Rad51, RPA, and Rad52 staining in wild-type and spo11 nuclei. The spo11 culture was split at 3 hr and 50% was irradiated with a dose of 100 krad (⋄). (A) Percent of nuclei that contain subnuclear foci. Counts are derived from images of 50–55 random nuclei from each time point. Nuclei were scored as staining positive if they contained at least five foci. Spores present on the slide were included in the calculation of total nuclei. (B) The average number of foci among staining positive nuclei in wild type and spo11. (C) Analysis of DNA content by FACS for wild type and spo11. Because preparation of spread nuclei requires an additional incubation of unfixed cells for at least 25 min at 30°C, the samples in this analysis are expected not to have progressed as far in the cycle as those used to prepare spread nuclei. (D) Analysis of meiotic divisions by fluorescent microscopy of DAPI-stained cells from wild type and spo11.

Time course analysis of Rad51, RPA, and Rad52 staining in wild-type and spo11 nuclei. The spo11 culture was split at 3 hr and 50% was irradiated with a dose of 100 krad (⋄). (A) Percent of nuclei that contain subnuclear foci. Counts are derived from images of 50–55 random nuclei from each time point. Nuclei were scored as staining positive if they contained at least five foci. Spores present on the slide were included in the calculation of total nuclei. (B) The average number of foci among staining positive nuclei in wild type and spo11. (C) Analysis of DNA content by FACS for wild type and spo11. Because preparation of spread nuclei requires an additional incubation of unfixed cells for at least 25 min at 30°C, the samples in this analysis are expected not to have progressed as far in the cycle as those used to prepare spread nuclei. (D) Analysis of meiotic divisions by fluorescent microscopy of DAPI-stained cells from wild type and spo11.