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(A) Anti-Rad51 immunostaining of mutant nuclei. Nuclei representative of those seen at 3–4 hr are shown. (B–C) Anti-RPA and anti-Rad52 immunostaining of rad51, rad51dmc1, rad52, rad55, rad57, and rad50S mutants at the times indicated. Sample nuclei with large numbers of intensely staining foci or aggregates typical of those seen at later hours are shown. The RPA staining example in late hour rad52 is a typical intermediate phenotype. Bar, 2 μm.
