Table 3.
Genetic requirements for focus formation II
| Straina
|
Probeb
|
||
|---|---|---|---|
| α-Rad51
|
α-Rad52
|
α-RPA
|
|
| wt | + | + | + |
| dmc1 | +++c | ++ | ++ |
| rad50S | + | N.D. | ++ |
| rad51 | (−)d | ++ | ++ |
| rad51dmc1 | (−) | ++ | ++ |
| rad52 | − | (−) | ++ |
| rad55 | − | ++ | ++ |
| rad57 | − | ++ | ++ |
| rad54 | + | N.D. | N.D. |
a
Strains used are wild-type (wt), NKY1314 and NKY1551; dmc1Δ, ASY103; rad50S, NKY1392; rad51Δ, ASY102; rad52Δ, DKB988xDKB992; rad54Δ, MSY241 or DKB1322; rad55Δ, D012; rad57Δ, D0018 or D006.
b
Antibodies as per Materials and Methods. (N.D.) Not determined.
c
(++) Brighter foci and/or accumulate at late hours in meiosis (see text).
d
[−] Negative control.