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. 1998 Jul 15;12(14):2114–2119. doi: 10.1101/gad.12.14.2114

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Copyright © 1998, Cold Spring Harbor Laboratory Press

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Mapping of interaction domains. α-HA coimmunoprecipitations of Myc-tagged Smad3 (A) or Smad4 (B) proteins with HA-tagged p300. (Input) Approximately 2% of Myc-tagged proteins in the cell extracts. Numbers at top refer to amino acids. (C) GST pull-down assays. Bound Myc-tagged Smad proteins were revealed by α-Myc Western blotting. (D) GST pull-down assay with 6HisT7–Smad3_210–425 followed by α-T7-tag Western blotting. (E) α-HA coimmunoprecipitations of Myc-tagged Smad2 proteins with p300–HA.

Mapping of interaction domains. α-HA coimmunoprecipitations of Myc-tagged Smad3 (A) or Smad4 (B) proteins with HA-tagged p300. (Input) Approximately 2% of Myc-tagged proteins in the cell extracts. Numbers at top refer to amino acids. (C) GST pull-down assays. Bound Myc-tagged Smad proteins were revealed by α-Myc Western blotting. (D) GST pull-down assay with 6HisT7–Smad3_210–425 followed by α-T7-tag Western blotting. (E) α-HA coimmunoprecipitations of Myc-tagged Smad2 proteins with p300–HA.