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. 1998 Jul 15;12(14):2114–2119. doi: 10.1101/gad.12.14.2114

Figure 4.

Figure 4

Figure 4

 Phosphorylation-dependent cooperation between Smad3 and CBP/p300. (A) 6Myc–Smad32–425 or 6Myc–Smad42–552 was coexpressed with TβRI–T204D where indicated. Immunoprecipitations were performed with α-p300 or α-CBP antibodies and coimmunoprecipitated Smad proteins revealed by α-Myc Western blotting. (B) Wild-type (wt) or the triple alanine mutant C3A of 6Myc–Smad32–425 was coexpressed with TβRI–T204D in 293 T cells, α-CBP immunoprecipitations of cell extracts were performed and Smad3 proteins were revealed by α-Myc Western blotting (lanes 1–4). Lanes 5–8 depict a corresponding in vitro pull-down experiment employing GST–CBP1891–2175. (C) Wild-type or C3A Myc–Smad3 expression plasmid (0.5 μg) was transfected with 6 μg of p300–HA and 0.5 μg TβRI–T204D vector into HeLa cells. Activation of the cotransfected 3TP–lux reporter gene is shown.