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. 1998 Jul 15;12(14):2144–2152. doi: 10.1101/gad.12.14.2144

Figure 2.

Figure 2

Figure 2

Figure 2

Figure 2

 Exchanging the L45 loops switches the signaling specificity of TβR-I and BMPR-IB. (A) Activation of the TGFβ-responsive reporter 3TP-luciferase in TβR-I-defective R1B/L17 cells transfected with wild-type or mutant receptors. Cells were incubated with TGF-β (T) or BMP2 (B), and luciferase activity was determined in triplicate samples. (Inset) HA-tagged receptors immunoprecipitated from metabolically labeled cells as controls. (B) Activation of the A3–CAT reporter containing activin- and TGF-β-responsive Mix.2 elements. R1B/L17 cells were transfected with Fast1 and receptor constructs. TβR-I transfectants were incubated with TGF-β and BMPR-IB transfectants with BMP2, and CAT activity was determined. (C) Activation of the BMP-responsive reporter Vent.2–luciferase in P19 cells transfected with TβR-II and wild-type or mutant TβR-I. Cells were incubated with BMP2 (B) or TGF-β (T), and luciferase activity was determined. (D) Induction of markers of dorsal mesoderm (muscle actin), ventral mesoderm (globin), and neural tissue (NRP-1) in Xenopus embryos. RNAs encoding the indicated constitutively active receptor forms were injected into the animal pole of two-cell embryos. Expression of muscle actin, globin, NRP-1, and EF-1α (as control) in animal caps from these embryos was determined. Animal caps from uninjected embryos (control), whole embryos (embryo), and a sample without reverse transcription (RT−) were included.

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