Table 2.
Experiment 1 |
Experiment 2 |
Experiment 3 |
Average pollen tube growth (%) | |||||||
---|---|---|---|---|---|---|---|---|---|---|
No. of pollen | No. pollen tubes | Pollen growth (%) | No. of pollen | No. of pollen tubes | Pollen growth (%) | No. of pollen | No. of pollen tubes | Pollen growth (%) | ||
Control | 98 | 0 | 0 | 105 | 0 | 0 | 126 | 0 | 0 | 0 |
La3+ | 84 | 48 | 57·1 | 84 | 53 | 63·1 | 137 | 81 | 59·1 | 59·8 ± 3** |
Verapamil | 205 | 77 | 37·6 | 144 | 49 | 34 | 167 | 71 | 42·5 | 38 ± 4* |
Calcimycin | 151 | 0 | 0 | 107 | 0 | 0 | 100 | 0 | 0 | 0 |
EGTA | 130 | 0 | 0 | 100 | 0 | 0 | 114 | 0 | 0 | 0 |
Ruthenium red | 105 | 0 | 0 | 126 | 0 | 0 | 100 | 0 | 0 | 0 |
Percentage pollen tube growth is given as mean ± s.d. of three replicate experiments. **P < 0·001; *P < 0·01 as determined by Student's t-test.