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Functional tests for T cells. Unseparated T cells from spleen and peripheral lymph nodes (pooled cervical and axillary lymph nodes) were evaluated for proliferative responses to various stimuli and readout taken at 570 nm following MTT assay (Promega) A. Proliferation assay with PMA+ionomycin; B. Proliefration to anti CD3/CD28 with cells from spleen, and C. with cells from the same lymph nodes as above.
