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ASF/SF2 depletion activates splicing of HIV-1 tat pre-mRNA. DT40–ASF cells stably transfected with an expression vector encoding HIV-1 tat pre-mRNA were incubated with tet as in Fig. 6. Total cellular RNA was isolated from these cells and analyzed by RNase protection. Scheme of tat pre-mRNA splicing is illustrated at the bottom. Numbers indicate the length of probe and protected fragments. (M) pBR322/HpaII marker.
