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PCR amplification followed by restriction fragment length polymorphism (PCR-RFLP) to determine MDM2 SNP309 polymorphism. MDM2 SNP309 T allele was not cleaved by MspAI endonuclease and had a single band of 174 bp. The MDM2 SNP309 G allele was cleaved by MspAI and had two small fragments of 126 and 48 bp. The MDM2 SNP309 heterozygote had three bands of 174, 126 and 48 bp.
