Figure 2.

SWI/SNF is recruited to DNA by activators independently of promoter sequences. PICs were assembled on both wild-type (W) and ΔPromoter (Δ) templates as stated in Fig. 1, with the nuclear extracts (NE) indicated. Reactions were performed either with no activator (−), the GAL4 (1–94) DNA-binding domain (G), or Gal4–AH (A) and Gal4–VP16 (V), as indicated. rTBP (400 ng) was added where indicated. PICs were analyzed by Western blotting with antibodies against the components indicated at right. (Lane 1) Dynabeads without templates were used in PIC assembly as a control for nonspecific binding to beads.