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. 1999 Sep 15;13(18):2388–2399. doi: 10.1101/gad.13.18.2388

Figure 4.

Figure 4

Effects of dominant-negative TERT mutants on 293 cells with long telomeres. (A) Western analysis of 293 wild-type clones (D1) and mutant clones A1 and A2 by the same method as described in Fig. 2. The expression of TERTs are indicated by arrowhead. (B) TRAP assay of the wild-type, A1, and A2 clones. (Lanes 7–10) The serial dilution of nontransfected 293 lysate. One microgram of each lysate was assayed. (C) TRF of wild-type, A1, and A2 clones over a period of 109 population doublings of growth in the presence of 2 μm muristerone. The same method as described in Fig. 2 was used. (D) Comparison of long-term growth of wild-type and mutant 293 clones . Cells were grown in the presence or absence of muristerone and passed every 3 days as described in Materials and Methods and counted per passage. Data represent population doublings of these clones in each passage over a 40-passage period. (□) 293WT; (♦); top, 293 A1; bottom, 293A2.