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. 2000 Nov 1;14(21):2725–2736. doi: 10.1101/gad.839400

Figure 4.

Figure 4

The RNase activity of hIRE1α is required for the mammalian UPR. (A) The reporter plasmids containing the luciferase gene under control of the rat BIP promoter and β-galactosidase under control of the SV40 early promoter were cotransfected with the hIRE1α expression plasmids into COS-1 cells as indicated. The transfected cells were treated with 10 μg/ml tunicamycin for 6 h prior to harvest. The luciferase activities are presented relative to SV40 β-galactosidase activities. Similar results were obtained from two independent experiments. (B) Cells were transfected as above in the presence of pMT2-μ or pMT2-Δsμ. The vector used for wild-type and mutant hIRE1α expression is pEDΔC.