Intact thymocyte development in RANK−/− mice. (A) Cell counts of thymi from 6- to 8-week-old RANK+/+ (n = 14) and RANK−/− (n = 14) mice. (B) The thymic weights were expressed as a proportion of total body weight. Note the equivalent thymic weight/body weight ratios observed in RANK+/+ and RANK−/− mice. (C) Flow cytometric analysis of thymi from 6-week-old RANK+/+ or RANK−/− mice. Thymocyte suspensions were stained for CD4 and CD8 (top) or gated, Lin− (CD3ε−, CD4−, CD8−, B220−, GR-1−, CD11b−, TCRγδ−) thymocyte precursors were stained for CD25 and CD44 (bottom).