Figure 1.
Chromosomal ARS301 conversion from pre- to postreplicative state in HU is blocked by the MEC1/RAD53 checkpoint. Genomic-footprinting analysis of the chromatin structure of ARS301. Wild-type (y300; left) or rad53 (y301; right (Allen et al. 1994) cells were arrested in G2 with nocodazole (NOC) or in G1 with α factor (α) or released from α-factor block into HU for 90 min (α → HU). Naked DNA (ND) was included as control. (*) ORC-induced hypersensitive sites. Cell cycle blocks and genomic footprinting protocols were as described previously (Santocanale and Diffley 1996, 1998). Samples were treated with increasing amounts of DNase I.