Table 2. Dissociation constants for human AZ-ODC and AZ-AZI complexes.
AZ peptide | K d,AZ-ODC (µM) | K d,AZ-AZI (µM) | ||||
WT | 0.71 | ± | 0.005 | 0.027 | ± | 0.0001 |
87-124 | 12.7 | ± | 0.28 | 0.16 | ± | 0.001 |
95-228 | 1.5 | ± | 0.01 | 0.024 | ± | 0.0002 |
95-176 | 5.6 | ± | 0.04 | 0.06 | ± | 0.004 |
95-135 | 10.3 | ± | 0.07 | ND | ||
95-124 | 18.6 | ± | 0.13 | 0.28 | ± | 0.002 |
100-228 | 5.3 | ± | 0.03 | 0.068 | ± | 0.0007 |
100-176 | 10.8 | ± | 0.07 | ND |
The dissociation constants (K d) of AZ-ODC and AZ-AZI were derived from global fitting of the sedimentation velocity data to the model of A+B↔AB hetero-association in the SEDTHAT program.