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. 2011 Sep 9;6(9):e24443. doi: 10.1371/journal.pone.0024443

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Culbertson et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A–F) EB ganglion rescue mediated by transplantation of wildtype NC into prdm1a^nl3 mutant embryos expressing Tg(NeuroD:EGFP). (A, B) Left (control) and right (transplanted) lateral views of prdm1a^nl3 mutant head with rhodamine-positive wildtype NC donor cells at 3 dpf. Tg(neuroD:EGFP)^nl1 expression reveals an absence of identifiable cranial ganglion structures on the non-transplanted side (A) while transplanted side shows restoration of these structures, including identifiable glossopharyngeal and vagal ganglia. (C, D) Distribution of rhodamine-positive donor NC cells shows an absence of fluorescent label in the cranial ganglion field of the non-transplanted side (C). In the transplant side (D), rhodamine-positive donor cells are present in parallel columns extending ventrally from the ganglion field (arrows) and in a single line perpendicular and immediately dorsal to columns (star), likely corresponding to the ceratobranchials and the rostral basicranial commisure, respectively. (E and F) Merged channel high-magnification views of control (E) and rescue (F) sides with DAPI counterstain (blue). Note DAPI-labeled branchial arches containing rhodamine-positive donor cells (brackets). (G–L) Partial EB ganglion rescue following transplantation of sox10^−/− NC cells into prdm1a^nl3 host embryos. (G,H) Tg(NeuroD:EGFP) expression in prdm1a^nl3 embryo showing absence of small EB ganglia in control side (G) and a partial rescue of ganglion formation in ventral vagal region of transplanted side (arrowhead). (I,J) Distribution of rhodamine-labeled donor cells in control side (I) and rescue side (J). (K,L) Merged channel high-magnification views of control (K) and rescue (L) sides with DAPI counterstain. Rescued side shows juxtaposition of rescued ganglion with the rhodamine-positive arches (brackets). Margins of statoacoustic ganglia are indicated by dashed white lines. Abbreviations: Tg/aLL/F = trigeminal/anterior lateral line/facial ganglion complex; SA = statoacoustic ganglion; pLLg = posterior lateral line ganglion; G = glossopharyngeal ganglion; V = vagal ganglia. Scale bar in (A) = 50 µm.