Figure 2.

Comparison of the proximal promoter regions of β-type globin genes. (A) Positions of distal (open box) or proximal (solid box) CAC boxes are shown relative to the CAAT box in human ɛ, γ, and β, and murine ɛy, b1, and b2 genes. For the human γ gene, the ^Aγ distal CAAT box and its upstream region was used for alignment. (B) Sequence alignment of human embryonic ɛ- and adult β-globin gene promoter regions as well as the mutant ɛ-globin promoters examined in this study (E + CAC and Bepsi). Mutated nucleotides in the two promoters are underlined. Positions corresponding to the distal CAC, proximal CAC, and CAAT boxes are bracketed. Putative binding sites for COUP–TFII in K562 and DRED in MEL cells are underlined with arrows.