Expression of dharma transcripts during embryogenesis. (A) Northern blotting analysis (15 μg of total RNAs were loaded). max was used as a loading control. (B–K) Whole-mount in situ hybridization of dharma (B–F) and its sagittal section (G–K). (B,G) High stage, (C,H) sphere stage, (D,I) dome stage, (E,J) 50% epiboly stage, (F,K) shield stage. (B–F) Lateral view. Right is dorsal. (L) gsc expression at the shield stage. (M,N) dharma expression at the sphere stage. Dorsal view. (M) Control embryo; (N) LiCl-treated embryo.