Table 2.
TEL−/− ES cells contribute to fetal liver hematopoiesis
| Chimera
|
Genotype
|
Red colonies
|
|||||
|---|---|---|---|---|---|---|---|
| (CFU-e)
|
(BFU-e)
|
Non-red Colonies
|
|||||
| −G418
|
+G418
|
−G418
|
+G418
|
−G418
|
+G418
|
||
|
|
+/−
|
510 ± 90
|
190 ± 45
|
70 ± 20
|
40 ± 5
|
910 ± 140
|
190 ± 30
|
| −/− | 655 ± 35 | 395 ± 50 | 110 ± 40 | 40 ± 20 | 750 ± 70 | 85 ± 10 | |
| Clone 1 | −/− | 610 ± 130 | 20 ± 10 | 115 ± 35 | 15 ± 5 | 925 ± 45 | 35 ± 10 |
| −/− | 770 ± 90 | 55 ± 10 | 170 ± 10 | 15 ± 5 | 1055 ± 165 | 35 ± 5 | |
| Clone 2 | −/− | 875 ± 75 | 360 ± 45 | 220 ± 20 | 90 ± 5 | 1200 ± 10 | 125 ± 35 |
| Clone 3 | −/− | --------------- N.D. ---------------- | 635 ± 35 | 85 ± 15 | |||
| −/− | 625 ± 45 | 50 ± 0 | |||||
One TEL+/− ES clone and three independently-derived TEL−/− ES cell clones were injected into wild-type blastocysts. Progenitor assays were performed on the resultant TEL/wild-type chimeric fetal livers (E14.5–E16.5). Erythroid (red) colonies, including CFU-e and BFU-e, and myeloid (non-red) colonies were cultured with or without G418 as in Fig. 2A. Numbers represent progenitors/106 fetal liver cells. (N.D.) Not determined.