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Wild-type CBF1, but not mutant CBF1 (EEF233AAA), derepresses GAL4–CBF1. GAL4–CBF1 was transfected along with increasing amounts of either wild-type CBF1 (lanes 2–6) or mutant CBF1 (EEF233AAA) (lanes 7–11) expression plasmids as indicated. Luciferase activity is normalized by β-galactosidase activity (described in Materials and Methods). The diagram illustrates how CBF1 overexpression may result in derepression of GAL4–CBF1.
