Figure 2.

LTB₄ and LTD₄ act via specific receptors to induce calcium in an additive manner. Monocytes were stimulated with different concentrations of LTB₄, LTD₄ or LTB₄+LTD₄ and calcium release was measured, as indicated in Methods (Figure 2A). Data presented as means ± SEM from 3–6 separate experiments each performed in triplicate. *p<0.05, analyzed by ANOVA. Representative traces of calcium flux induced by repeated exposure to LTD₄ (10 nmol/L) and LTB₄ (10 nmol/L) in monocytes (B and C). Monocytes were pretreated with MK (20 nmol/L) or CP105696 (10 nmol/L) for 20 min and stimulated with LTD₄ (100 nmol/L) (D), or LTB₄ (10 nmol/L) (E). The effect of treatment with both LTB₄ and LTD₄ was completely inhibited by pretreatment with both inhibitors (F). Data from one of three separate experiments, each with similar results, are shown.