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. Author manuscript; available in PMC: 2012 Sep 30.
Published in final edited form as: J Immunol Methods. 2011 Jul 18;372(1-2):22–29. doi: 10.1016/j.jim.2011.06.026

Fig. 1. Primary CD8+ cell transfection efficiency under different conditions.

Fig. 1

Primary human CD8+ cells were freshly isolated using Dynal or Miltenyi immunomagenetic (IM) beads from normal buffy coats and washed once with phosphate buffered saline (PBS). 200,000 cells were electroporated in 10 μl tips in Buffer T with 1 μl of 1 μg/μl GFP plasmid. The transfection efficiency was tested by flow cytometery (BD FACsort), 24 hours after electroporation. Transfection conditions were varied to compare the effect of different voltages, pulse widths (10 ms, 20 ms or 30 ms) and pulse repetitions (once or twice) on transfection efficiency. This figure shows transfection efficiency of CD8+ cells isolated using Dynal beads.