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. Author manuscript; available in PMC: 2012 Sep 30.
Published in final edited form as: J Immunol Methods. 2011 Jul 18;372(1-2):22–29. doi: 10.1016/j.jim.2011.06.026

Fig. 2. Confocal imaging of GFP expression in primary human CD8+ cells.

Fig. 2

Two million CD8+ cells were transfected with GFP plasmid or transfection buffer. After removal of dead cells, the nuclei of CD8+ cells were stained with Hoechst33342 (2 μg/ml) and cells were fixed with 4% paraformaldehyde (PFA). Confocal photos were taken using 20x and 40xZoom4x (low right corner) lens. A-D, primary human CD8+ cells were transfected with buffer as control. E-H, primary human CD8+ cells were transfected with GFP plasmid under 20ms/Pulse1/2200V conditions.