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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: Mol Cancer Ther. 2011 Jun 23;10(9):1698–1708. doi: 10.1158/1535-7163.MCT-11-0107

Figure 3.

Figure 3

E3330 inhibits the activity of NFκB, HIF1, and AP-1 in a cell-based reporter assay. A – C, Panc-1 and PaCa-2 cells were transfected with NFκB, HIF1, or AP-1–Luc construct and cotransfected with a Renilla vector, pRL-TK. After 16-h, cells were treated with E3330 for 24-h, and Firefly and Renilla luciferase activities were assayed using Renilla luciferase activity for normalization. All transfection experiments were performed in triplicate and repeated at least 4 times in independent experiments. Data are expressed as Relative Luciferase Units (RLU) normalized to DMSO showing the mean ± SE. Student’s t tests were performed, and * p < 0.05 level comparing DMSO to E3330. D) qPCR analysis of CA-IX expression following E3330 treatment and hypoxia. Expression in Panc-1 cells was normalized to DMSO in normoxia (left). However, basal levels of CA-IX were undetectable in PaCa-2 cells in normoxia, therefore the CA-IX expression was normalized to DMSO under hypoxia (right). Shown is one representative experiment. Samples were run in triplicate and experiment performed in both cell lines in duplicate.