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. 2011 Aug;34(4pt1):379–389. doi: 10.1111/j.1365-2605.2010.01091.x

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International Journal of Andrology © 2011 European Academy of Andrology

Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.

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Immunofluorescent terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labelling (TUNEL) staining of serum- and glucocorticoid-inducible kinase-1 (SGK1)/TUNEL, p-FOXO3a Thr³²/TUNEL and FOXO3a/TUNEL in the ipsilateral 4-h testis sections. The apoptosis cells (arrows) are pinpointed by TUNEL stain (green; A, E, and I), whereas the expression of SGK1 (purple; B) and p-FOXO3a Thr³² (red; F) is barely detectable. The expression of FOXO3a (red; J) is not directly relatable to identifiable apoptotic cells. The colocalization images of SGK1/TUNEL, p-FOXO3a Thr³²/TUNEL and FOXO3a/TUNEL are shown in C, G and K. The sections are counterstained with DAPI (blue; D, H and I). Scale bar: 20 μm.