Homologous recombination in T. brucei RAD51 and RAD51 paralogue mutants. A. The number of transformants generated (per 106 cells put on antibiotic selection) when the construct tub-BLE-tub was electroporated into wild-type (wt) T. brucei, or into two independent heterozygous (+/− 1, 2) or homozygous (−/− 1, 2) mutants of RAD51-4 or RAD51-6, is shown. B. A comparison of the transformation frequency of wt, rad51−/−, rad51-3−/−, rad51-4−/−, rad51-5−/− and rad51-6−/− cells. Values are averages from at least three experiments; bars indicate standard deviation.