Fig. 5.

Effect of RAD51 or RAD51 paralogue mutation on the frequency of T. brucei VSG switching. A. Analysis of the effect if RAD51-4 or RAD51-6 mutation on VSG switching. The frequency with which VSG switch variants arose is shown in wild-type T. brucei cells (strain 3174.2; wt), two independent heterozygous (+/− 1, 2) or homozygous mutants (−/− 1, 2), and in −/− cells re-expressing the mutated gene (−/−/+). B. A comparison of VSG switching frequency in wt, rad51−/−, rad51-3−/−, rad51-4−/−, rad51-5−/− and rad51-6−/− cells. Values are the means of at least three independent experiments, and bars indicate standard deviation.