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. 2011 May 25;81(2):434–456. doi: 10.1111/j.1365-2958.2011.07703.x

Fig. 7.

Fig. 7

Interactions and expression interdependency of T. brucei RAD51 paralogues in vivo. A. RAD51-3 and RAD51-4 interact in vivo. Affinity-purified polyclonal antiserum against RAD51-3 or RAD51-4 was used to immunoprecipitate (IP) cognate protein from wild-type (wt) and rad51-3−/− or rad51-4−/− cells respectively. The presence or absence of RAD51-3 and RAD51-4 in all cases is shown by Western blots (WB) of the precipitated proteins, after SDS-PAGE, using anti-RAD51-3 and anti-RAD51-4 antiserum. B. Western blots are shown of whole cell extracts, after SDS-PAGE, from wt cells and from homozygous mutants (−/−) and re-expressers of RAD51-3, RAD51-4 and RAD51-6. Expression of RAD51-3 (51-3) or RAD51-4 (51-4) was assayed using affinity-purified polyclonal antiserum; a cross-reacting band is indicated in the anti-RAD51-3 blot (*), and a larger band seen only in the RAD51-4 re-expresser cell probed with anti-RAD51-4 antiserum is indicated (51-4Rex). As a loading control, the blots were stripped and re-probed with anti-OPB antiserum (gift, J. Mottram).