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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: Mol Cancer Ther. 2011 Jun 22;10(9):1740–1750. doi: 10.1158/1535-7163.MCT-11-0309

Figure 4. Nab3 inactivation confers UC1 sensitivity.

Figure 4

(A) Schematic of readthrough detection. See text for details. (B) Triplicate cultures of erg6Δ and erg6Δ ira2Δ were treated with DMSO or UC1 for 8 hours. Strain growth was monitored by OD600 and converted to cells per mL. Values are the mean +/− standard deviation of three cultures. (C) RT-PCR detection of readthrough transcripts in nab3-3 and UC1-treated samples. Samples from cells treated in (B) were processed for RNA extraction and RT-PCR as described in Materials and Methods. The presence of product indicates readthrough transcription at the SNR locus.