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. Author manuscript; available in PMC: 2012 Sep 30.
Published in final edited form as: J Immunol Methods. 2011 Jul 18;372(1-2):65–70. doi: 10.1016/j.jim.2011.06.031

Figure 3. Immunodepletion of Ara h 2, Ara h 6, and both.

Figure 3

Immunoblots are shown probed with a combination of rabbit anti-Ara h 1, 2, and 6 antibodies and developed with anti-rabbit IgG (A) and probed with the human pooled serum developed with anti-human IgE (B). Each lane contained 1µg (A) or 2 µg (B) of CPE following passage over columns containing both anti-Ara h 2 and anti-Ara h 6 (lane 1), anti-Ara h 6 alone (lane 2), anti-Ara h 2 alone (lane 3), or pre-immune IgG (lane 4). For this figure, the ratio of CPE to packed beads was ~4.5 mg of CPE to 1 ml of packed beads containing ~4 mg of affinity purified antibody.

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