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. Author manuscript; available in PMC: 2012 Sep 30.

Published in final edited form as: J Immunol Methods. 2011 Jul 18;372(1-2):65–70. doi: 10.1016/j.jim.2011.06.031

RBL-AB cells were sensitized with IgE from a pool of sera from highly peanut-allergic subjects as described in the materials and methods section and stimulated with CPE depleted of Ara h 2 alone (A), depleted of Ara h 6 alone (B) and depleted of both Ara h 2 plus Ara h 6 (C) as shown in Figure 3. Solid symbols are the CPE treated with pre-immune serum and open symbols are allergen depleted CPE. In each experiment (A, n=3; B, n=3; C, n=7), cells were stimulated in triplicate with a maximal amount of CPE (200 mg/ml). In order to pool the experimental results, the data are expressed as a percent of the maximal net (total minus background) degranulation in each assay. Maximal net degranulation with CPE (mean±SEM): A, 41±5%; B, 42±4%; C, 40±2%. If the standard error bars are not visible, they were smaller than the symbol. Best-fit lines were generated using a one-phase decay model (Prism 5.0c for MacIntosh; GraphPad Software, Inc. La Jolla, CA).

RBL-AB cells were sensitized with IgE from a pool of sera from highly peanut-allergic subjects as described in the materials and methods section and stimulated with CPE depleted of Ara h 2 alone (A), depleted of Ara h 6 alone (B) and depleted of both Ara h 2 plus Ara h 6 (C) as shown in Figure 3. Solid symbols are the CPE treated with pre-immune serum and open symbols are allergen depleted CPE. In each experiment (A, n=3; B, n=3; C, n=7), cells were stimulated in triplicate with a maximal amount of CPE (200 mg/ml). In order to pool the experimental results, the data are expressed as a percent of the maximal net (total minus background) degranulation in each assay. Maximal net degranulation with CPE (mean±SEM): A, 41±5%; B, 42±4%; C, 40±2%. If the standard error bars are not visible, they were smaller than the symbol. Best-fit lines were generated using a one-phase decay model (Prism 5.0c for MacIntosh; GraphPad Software, Inc. La Jolla, CA).

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