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. Author manuscript; available in PMC: 2012 Sep 30.
Published in final edited form as: J Immunol Methods. 2011 Jul 18;372(1-2):71–77. doi: 10.1016/j.jim.2011.06.033

Figure 2. Recovery of spiked cytokines from subject samples by MSD and CBA.

Figure 2

Figure A shows mean percent recovery of IL-6 added at final concentrations of 100, 50, 25, 12.5, and 6.25 pg/ml to serum samples of three HIV-negative (solid line) and nine HIV-positive donors (dashed line), as measured by the MSD (left) and CBA (right) kits. Figures B to F show the mean recovery of IL-6, IL-10, IL-8, IL-12p70, TNF-α, and IL-1β among all study participants obtained with the MSD (solid line) and CBA (dashed line) kits, regardless of HIV infection, AIDS, or therapy status (N=12). Horizontal gray lines show the 100 ± 25% acceptance criteria. Error bars show ± 1 standard error of the mean (SEM) of the mean values.