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. 1998 Aug 1;12(15):2381–2391. doi: 10.1101/gad.12.15.2381

Figure 3.

Figure 3

Figure 3

Figure 3

 Physical map of the ROT3 locus and transcription of the wild-type gene. (A) Schematic map of the T-DNA-tagged rot3 gene. An arrow indicates the promoter sequence of the ROT3 gene. Recognition sites for restriction endonucleases BamHI (B), EcoRI (E), and HindIII (H) are shown on the map. (B) Southern genomic DNA hybridization analysis of wild-type (wt) and rot3 alleles. Genomic DNA was digested with EcoRI and fragments after electrophoresis were allowed to hybridize with probe D shown in A. The hybridization pattern of rot3-1 indicates a deletion in the promoter and the first exon–intron region of the ROT3 locus. (C) RNA blot hybridization analysis of the wild-type plant 4 weeks after sowing. Hybridization of 1.5 μg of poly(A)+ RNA with probe B shown in A. The result indicated that the transcribed RNA was 1.8 kb long. The membrane was exposed to an imaging plate for 1 week, and the band was visualized by an image analyzer (BAS1000; Fujix, Tokyo, Japan).