Figure 6.

 Amplification by RT–PCR of ROT3 mRNA with primers pROT3-1 and pROT3-2. Total RNA was used for RT–PCR. Amplified DNAs were allowed to hybridizae with ³²P-labeled ROT3 cDNA with high-stringency conditions for hybridization and washing. Arrows indicate 0.28-kb products of PCR. (A) Expression of wild-type and rot3 alleles. Total RNA was isolated from 4-week-old leaves. One microgram of total RNA was used in each case. (B) Expression of the mRNA in various tissues of Arabidopsis. Total RNA was isolated from suspension cultured cells, 7-day-old seedling, root, cotyledon, rosette leaf, stem, and floral bud. Two micrograms of total RNA was used in each case. Result for amplification by RT–PCR of β-tubulin4 (TUB4) fragments was shown for standard controls (B; bottom).