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. Author manuscript; available in PMC: 2011 Sep 10.
Published in final edited form as: Physiol Res. 2010 Apr 20;59(6):927–935. doi: 10.33549/physiolres.931979

Fig. 3.

Fig. 3

ATP dose-response curves for single and double threonine mutants of P2X4R. Concentration dependence of ATP on the peak current amplitude responses in single-point mutants (left and middle panels) and double mutants (right panels) for five disulfide bonds is shown in the absence (open circles) and presence (closed circles) of 3 μM IVM. Each row shows paired cysteine mutants that form disulfide bonds (SS1-5). Vertical and horizontal dashed lines (in blue) indicate the EC50 and Imax values, respectively, for P2X4R-WT in the presence of 3 μM IVM. The vertical dotted lines (in black) represent the mean EC50 values for T-mutants in the presence of IVM and significant shifts (red arrows). Data shown are mean ± SEM from 6–30 cells.