Ultra-structural changes of a pollen mother cell (PMC) before and during meiosis in the hybrids of autotetraploid rice. (a–d, f) Normal meiosis in diploid rice (CK). (g–t) Abnormal meiosis in the hybrids of autotetraploid rice with a low setting rate; Ta, tapetum; V, vacuole. Bars are 1 μm in (j), (l), (p), and (q), 2 μm in (h),and all other bars are 5 μm. (a) Normal PMC at early interphase; (b) normal PMC at interphase; (c) normal PMC at leptotene; (d) normal PMC at pachytene; (e) normal dyad of Guanglu'ai 4-4x; (f) normal tetrad showing two cells. The other two cells are another section and are not shown. (g) Abnormal PMC at early interphase; vacuoles formed in the PMC; (h) magnification of (g) showing vacuoles in one PMC; (i) abnormal PMC at interphase showing many vacuoles in the PMC; (j) magnification of (i) showing the vacuoles in one PMC; (k) abnormal PMC at leptotene and plenty of vacuoles and abnormal organelles are present in the abnormal PMC; (l) magnification of (k) showing the vacuoles and abnormal organelles in the PMC; (m) leptotene showing the abnormal PMC; (n) zygotene with degraded PMC; (o) pachytene showing the abnormal nucleus of the PMC (arrow); (p) magnification of (o) and the nuclear membrane of PMC has degenerated (arrows); (q) pachytene where the nucleus and cytoplasm are both abnormal at the same time. Arrows indicate the abnormal nucleus of the PMC. (r) Abnormal proliferating tapetum. (s) Abnormal dyad, one has degenerated (arrow). (t) Abnormal tetrad.