Fig. 10.

Fluorescence microscopic imaging of NO in the proximal (junction of pedicel) fracture plane of the AZ, located between the pedicel and fruit pericarp, from ARB (A, C, E) and PIC (B, D, F) olive fruit at 217 DPA were incubated in MES-KCl buffer alone (A, B; control), with DAF-FM-DA as fluorescent probe (C, D), or in combination with cPTIO (E, F), and examined by confocal laser scanning microscopy. The presence of NO is shown by green fluorescence, which is distinguishable from autofluorescence, showed here in red. Co-localization of both fluorescence sources results in yellow colour. Experiments were repeated at least five times with similar results. Scanning electron micrographs (SEMs) of the proximal fracture plane of the olive fruit AZ from an ARB (G) and PIC (H) cultivar. co, cortex; ep, epidermis. pi, pith; xy, xylem. Scale bars: 250 μm in A–F; 20 μm in G and H.