Table 2.

Relative levels of NO in the olive fruit AZ–AC after treatments

Cultivar		Control	ET	Put	Spd	AOA	CoCl2	CHA	MGBG
ARB
	7 d	1.0	0.5*	1.0	1.0	1.2	1.0	0.4*	1.0
	14 d	1.0	0.5*	1.1	1.0	1.2	1.0	0.5*	0.8
PIC
	7 d	1.0	0.1*	1.2	1.0	1.4*	1.0	0.8	1.0
	14 d	1.0	0.1*	1.2	2.3*	1.1	3.8*	0.1*	0.1*

Olive trees (ARB and PIC cultivars) were sprayed with water (control), 10 mM ethephon (ET), 1 mM putrescine (Put), 1 mM spermidine (Spd), 10 mM aminooxyacetic acid (AOA), 10 mM cobalt chloride (CoCl2), 10 mM cyclohexylamine (CHA), or 1 mM methylglycoxalbis-guanylhydrazone (MGBG), and the fruit AZ–AC were then harvested 7 d and 14 d after spraying. NO content was measured as relative fluorescence units (RFU) using DAF-FM-DA dye and a fluorometer (n=5, ±SE), and the fold ratio is calculated with the corresponding control sample (water-treated sample). The experiment was repeated three times with similar results, and data for one representative experiment are shown. Asterisks indicate values that were determined by the t-test to be significantly different (P <0.05) from control.