Fig. 5.

Immunoblotting and fluorescence intensity quantification from tobacco leaf epidermal cells expressing γ-gliadin or its domains. (A) An immunoblot was performed with anti-GFP antibodies. M, protein marker; Y, tobacco leaf infiltrated with a YFP construct as a positive control; C, tobacco leaf expressing C-terminal γ-gliadin–GFP; N, tobacco leaf expressing N-terminal γ-gliadin–GFP; FL, tobacco leaf expressing full-length γ-gliadin–GFP. (B) Fluorescence intensity quantification from tobacco leaves expressing the fusion proteins. Fluorescence intensities were measured from confocal images of tobacco leaves expressing full-length γ-gliadin–GFP, Nter-γ-gliadin–GFP, or Cter-γ-gliadin–GFP proteins. They are expressed as the grey level mean per pixel.