Figure 5.

Regulation of SREBP-1 protein expression by rexinoid. Immunoblot analysis of SREBP-1 and SREBP-2 was performed on fractionated cell extracts from liver and intestine of wild-type male mice fed diets containing 0.2% cholesterol plus vehicle (−) or 30 mpk LG268 (+) for 18 h. P and N denote the precursor and cleaved nuclear forms of SREBP, respectively, and the asterisk indicates a nonspecific band. The filters for liver and intestine were exposed to film at room temperature for 15 sec and 45 sec, respectively.