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. 2000 Nov 15;14(22):2819–2830. doi: 10.1101/gad.844900

Figure 8.

Figure 8

Mutation of the LXRE sequence abolishes activation of mouse SREBP-1c promoter by RXR/LXR. (A) Schematic diagrams of pBP1c(6500)-Luc wild-type and mutant reporter plasmids. Differences between wild-type and mutant plasmids (nucleotides −300 to −280) are shown for comparison. (B) Activation of wild-type, but not mutant, LXRE reporter plasmids. Wild-type and mutant pBP1c(6500)-Luc were cotransfected into HEK-293 cells. For each 60 mm dish 0.5 μg pBP1c-Luc, 25 ng, CMV-βgal and CMX-LXRα or pCDNA3 were used. Three hours after transfection, cells were switched to medium A containing vehicle (DMSO) or 10 μM T0901317, and assayed as described in Figure 6. Data represent three independent transfection experiments (each in duplicate).