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. 2011 Jun 3;62(13):4627–4637. doi: 10.1093/jxb/err183

Fig. 2.

Fig. 2.

Contents of adenosine and 2′3′-cAMP in leaves and isolated vacuoles of ENT1 mutants and release of adenosine from liposomes reconstituted with vacuolar membrane protein. (A) Adenosine contents in leaves from WT, 35S:ENT1 overexpresser lines, and ENT1-RNAi lines; (B) adenosine contents in isolated vacuoles from WT, 35S:ENT1 over-expresser line #9, and ENT1-RNAi line #42; (C) 2'3'-cAMP contents in isolated vacuoles from WT, 35S:ENT1 over-expresser line #9, and ENT1-RNAi line #42; (D) release of [14C]-adenosine from preloaded liposomes reconstituted with vacuolar membrane protein from WT (circles), 35S:ENT1 over-expresser line #9 (squares), and ENT1-RNAi line #38 (triangles). All data shown represent the mean of at least three independent experiments (6SE). The asterisks indicate significant differences between WT and mutants, based on Student's t test (*P <0.05, **P <0.01, ***P <0.005).