Fig. 5.

Pharmacological and molecular evidence showing that BnHO1 is required for salinity and osmotic stress-induced LR formation. Three-day-old rapeseed seedlings were treated with distilled water (Con), 10 mM or 100 mM NaCl (S10 or S100), 2% or 20% PEG (P2 or P20), 50 μM ZnPPIX, 10% saturated CO aqueous solution (CO), 1 μM haemin alone, or the combination treatments for another 3 d. Representative photos were then taken. Bar, 1 cm (A). Meanwhile, the length (>1 mm) and number of LRs per seedling were determined after 3 d of treatment (n=90, B). BnHO1 transcripts (C) and HO activity (D) were analysed after 6 h of various treatments. Data are the means ±SE of at least three independent experiments. Within each set of experiments, bars denoted by the same letter did not differ significantly at the P<0.05 level according to Duncan's multiple test. ND, none detected.