Fig. 3.
Stimulation of cyclic GMP accumulation by cannabinoid drugs in N18TG2 cells. N18TG2 cells were treated for 5 min with 1 μM CP55940 (CP) or WIN55212-2 (WIN) in the presence of 0.1 mM IBMX plus 0.1 mM rolipram. ODQ was added at 0.3 μM where indicated. Basal values of cyclic GMP (PSS alone) were subtracted, and data are reported as the mean ± SEM from N=3 cell dishes. *Significantly greater than IBMX + rolipram at p<0.05; **significantly lower than drug-stimulated value in the absence of ODQ, but not significantly different from IBMX+rolipram+ODQ at p<0.05 by Student’s t-test. This experiment was representative of four similar experiments having qualitatively similar results.