Fig. 6.

Attenuation of the cannabinoid drug-dependent depletion of GC-β₁ from the cytosol by the CB₁ antagonist rimonabant or pertussis toxin. N18TG2 cells were treated with 1 μM rimonabant (30 min) or 100 ng/ml pertussis toxin (16 h) followed by incubation with the vehicle control, 1 μM CP55940, or 1 μM WIN55212-2 for 1 h. Cytosolic preparations and Western blotting were performed as described in the Methods. Densitometry was performed, and data were normalized to control as 100 %. For rimonabant treatment groups, data from N=3 individual Western blots are shown as the mean ± SEM, and analyzed by Prism using one-way ANOVA, with no significant differences found between treated and vehicle control samples. For the pertussis toxin treatment, densitometry was performed on N=2 individual Western blots, and data are shown as the range of the values.