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. 2011 Aug 12;7:47. doi: 10.1186/1746-6148-7-47

Figure 1.

Figure 1

Real-time quantification of DNA extracted from MmmSC cultures. DNA concentration was measured spectrophotometrically and adjusted to 100 ng μl-1. Five tenfold dilution series were prepared and applied in duplex rt-PCR assays comprising the amplification and detection of the targets MSC_0136 (A) and MSC_1046 (B) with the TaqMan® LNA probes FAM-MSC_1046 and Cy5-MSC_0136, respectively. The detection limits are 10 fg DNA and 100 fg DNA corresponding to approximately 8 genome equivalents and 80 genome equivalents for the MSC_0136 and MSC_1046 assay, respectively.