Table 1.
Specificity testing of rt-PCR assays with isolates of the M.mycoides cluster (A), mollicute strains frequently isolated from bovine lungs (B) and bacterial strains causing CBPP-like symptoms (C)
| Species | Strain | n | Host | Origin | Year | MSC_0136 rt-PCR | MSC_1046 rt-PCR | |
|---|---|---|---|---|---|---|---|---|
| A | M. mycoides | PG1T | cattle | ? | ? | + | + | |
| subsp. Mycoides SC | diverse | 19 | cattle | Italy | 1990-1992 | + | + | |
| diverse | 3 | cattle | Spain | unknown | + | + | ||
| P0 | cattle | France | 1967 | + | + | |||
| N6 | cattle | Botswana | 1996 | + | + | |||
| M375 | cattle | Botswana | 1995 | + | + | |||
| Afade | cattle | Cameroon | 1968 | + | + | |||
| PO2 | cattle | France | 1980 | + | + | |||
| L2 | cattle | Italy | 1993 | + | + | |||
| Fatick | cattle | Senegal | 1968 | + | + | |||
| C11 | cattle | Chad | 1962 | + | + | |||
| 95014 | cattle | Tanzania | 1995 | + | + | |||
| T1/44 | cattle | Tanzania | 1952 | + | + | |||
| Gladysdale | cattle | Australia | 1953 | + | + | |||
| Shawawa | cattle | Botswana | 1995 | + | + | |||
| 2091 | cattle | France | 1984 | + | + | |||
| 197 | cattle | Italy | 1992 | + | + | |||
| B103 | cattle | Portugal | 1986 | + | + | |||
| 6305 | cattle | Portugal | 1993 | + | + | |||
| 6526 | cattle | Portugal | 1993 | + | + | |||
| Astercous | cattle | Spain | 1987 | + | + | |||
| Madrid | cattle | Spain | 1993 | + | + | |||
| Tan8 | cattle | Tanzania | 1998 | + | + | |||
| Matapi | cattle | Namibia | 2004 | + | + | |||
| Mandigwan | cattle | Namibia | 2001 | + | + | |||
| V5 | cattle | Australia | 1936 | + | + | |||
| IS31 | cattle | Tanzania | 1998 | + | + | |||
| 2022 | cattle | France | 1984 | + | + | |||
| B237 | cattle | Kenya | 1987 | + | + | |||
| M. mycoides | PG3, NCTC 10137T | goat | Turkey | 1950 | - | - | ||
| subsp. capri | Y-Goat | goat | Australia | 1956 | - | - | ||
| 7302 | goat | Portugal | < 1994 | - | - | |||
| Kombolcha | goat | Ethiopia | 1975 | - | - | |||
| 7730 | goat | France | < 1997 | - | - | |||
| 9096-C9415 | goat | Nigeria | unknown | - | - | |||
| diverse | 6 | goat | Gran Canaria | 1993 | - | - | ||
| My-I | goat | Croatia | 1986 | - | - | |||
| D2482 | goat | Switzerland | 1991 | - | - | |||
| 95010 | goat | France | 1995 | - | - | |||
| 6443-90 | goat | France | 1990 | - | - | |||
| My-325 | goat | Croatia | 1986 | - | - | |||
| C260/4 | goat | Gran Canaria | 1993 | - | - | |||
| Wk354 | goat | Switzerland | 1980 | - | - | |||
| L | goat | France | 1975 | - | - | |||
| Wi18079 | goat | Germany | 2009 | - | - | |||
| diverse | 3 | Barbary sheep | Germany | 1994 | - | - | ||
| M-5 | goat | Croatia | 1988 | - | - | |||
| M-18 | goat | Croatia | 1988 | - | - | |||
| M-29 | goat | Croatia | 1988 | - | - | |||
| M. capricolum | Cal. kid, NCTC 10154T | goat | USA | 1954 | - | - | ||
| subsp. capricolum | C47 | sheep | Germany | < 1992 | - | - | ||
| 4146 | goat | France | 1980 | - | - | |||
| 7714 | goat | France | 1967 | - | - | |||
| 609-79 | goat | France | 1979 | - | - | |||
| 8086-1 | goat | France | 1980 | - | - | |||
| M. capricolum | F38, NCTC 10192T | goat | Kenya | 1976 | - | - | ||
| subsp. capripneumoniae | 87F05 | goat | Turkey | 2005 | - | - | ||
| diverse | 2 | gazelle | UAE | 2004 | - | - | ||
| 3535 | mouflon | Qatar | 2005 | - | - | |||
| GL97P | goat | Tunisia | 1980 | - | - | |||
| M. leachii | PG50, NCTC 10133T | cattle | Australia | 1963 | - | - | ||
| D318b | cattle | Germany | unknown | - | - | |||
| D424 | cattle | Germany | unknown | - | - | |||
| Calf1 | cattle | Nigeria | unknown | - | - | |||
| M.nov.spec. | 8756-13 | mountain goat | USA | 1987 | - | - | ||
| G1650 | capricorn | Germany | 1994 | - | - | |||
| G5847 | capricorn | Germany | 1993 | - | - | |||
| B | M. putrefaciens | KS1, NCTC 10155T | goat | USA | 1955 | - | - | |
| 7578.94 | goat | France | 1994 | - | - | |||
| Tours2 | goat | France | 1972 | - | - | |||
| M. bovis | PG45, NCTC 10131T | cattle | USA | 1962 | - | - | ||
| 363/88 | cattle | Germany | 1988 | - | - | |||
| 334/96 | cattle | Germany | 1996 | - | - | |||
| 54/10 | cattle | Germany | 2010 | - | - | |||
| M. bovirhinis | PG43, NCTC 10118T | cattle | UK | 1967 | - | - | ||
| 320/93 | cattle | Germany | 1993 | - | - | |||
| 19/94 | cattle | Germany | 1994 | - | - | |||
| 365/09 | cattle | Germany | 2009 | - | - | |||
| M. bovigenitalium | PG11, NCTC 10122T | cattle | UK | 1947 | - | - | ||
| 338/87 | cattle | Germany | 1987 | - | - | |||
| 18/92 | cattle | Germany | 1992 | - | - | |||
| 466/94 | cattle | Germany | 1994 | - | - | |||
| M. arginini | G230, NCTC 10129T | mouse | USA | 1968 | - | - | ||
| 162/81 | cattle | Germany | 1981 | - | - | |||
| 208/94 | Barbary sheep | Germany | 1994 | - | - | |||
| 23/05 | sheep | Germany | 2005 | - | - | |||
| M. californicum | St-6, NCTC 10189T | cattle | USA | 1981 | - | - | ||
| 456/83 | cattle | Germany | 1983 | - | - | |||
| 111/84 | cattle | Germany | 1984 | - | - | |||
| M. canadense | 275C, NCTC 10152T | cattle | Canada | 1974 | - | - | ||
| 380/94 | cattle | Germany | 1994 | - | - | |||
| M. verecundum | 107, NCTC 10145T | cattle | UK | 1974 | - | - | ||
| 49/94 | cattle | Germany | 1994 | - | - | |||
| M. alkalescense | PG51, NCTC 10135T | cattle | Australia | 1961 | - | - | ||
| 26/93 | cattle | Germany | 1993 | - | - | |||
| M. canis | PG14, NCTC 10146T | dog | UK | 1951 | - | - | ||
| 306/93 | dog | Germany | 1993 | - | - | |||
| A. laidlawii | PG8, NCTC 10116T | unknown | UK | 1967 | - | - | ||
| 54/99 | cattle | UK | 1999 | - | - | |||
| A. axanthum | S743, NCTC 10138T | tissue culture | UK | 1970 | - | - | ||
| 824/80 | cattle | Germany | 1980 | - | - | |||
| Ureaplasma diversum | A417, NCTC 10182T | cattle | UK | 1972 | - | - | ||
| C | Pasteurella multocida | ATCC 43137T | pig | - | - | |||
| Mannheimia haemolytica | ATCC 33396T | unknown | - | - | ||||