Table 2.

Results of increasing starting DNA on real-time PCR assay performance.

	Sample
	01916a		4022a		4477b		01870a		04034a
DNAc	IS6110_T	β-actin	IS6110_T	β-actin	IS6110_T	β-actin	IS6110_T	β-actin	IS6110_T	β-actin
100	+	+	-	+	-	+	-	+	-	+
250	+	+	+	+	-	+	-	+	-	+
500	+	+	+	+	-	+	-	+	-	+
1000	+	+	+	+	-	+	-	+	-	+

^a M. bovis infected tissues

^b M. smegmatis infected tissues

^c DNA isolated from homogenized tissues added to PCR reaction in nanograms.